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ProductsBack/ICAM1 / CD54 antibody
WB analysis of various samples using GTX66707 ICAM1 / CD54 antibody. Lane1 : HEK293T whole cell lysate(20ug) Lane2 : A549 whole cell lysate(40ug) Lane3 : H1792 whole cell lysate(40ug) Lane4 : C6 whole cell lysate(40ug) Lane5 : SP2/0 whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66707 ICAM1 / CD54 antibody. Lane1 : HEK293T whole cell lysate(20ug) Lane2 : A549 whole cell lysate(40ug) Lane3 : H1792 whole cell lysate(40ug) Lane4 : C6 whole cell lysate(40ug) Lane5 : SP2/0 whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66707 ICAM1 / CD54 antibody. Lane1 : HEK293T whole cell lysate(20ug) Lane2 : A549 whole cell lysate(40ug) Lane3 : H1792 whole cell lysate(40ug) Lane4 : C6 whole cell lysate(40ug) Lane5 : SP2/0 whole cell lysate(40ug) Dilution : 1:500

ICAM1 / CD54 antibody

GTX66707
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetICAM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ICAM1 / CD54 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3383
  • Target name
    ICAM1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    BB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05362
  • Protein Name
    Intercellular adhesion molecule 1
  • Scientific Description
    This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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