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IHC-P analysis of human kidney tissue using GTX84306 IGF2BP2 antibody [3F9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of human kidney tissue using GTX84306 IGF2BP2 antibody [3F9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of human kidney tissue using GTX84306 IGF2BP2 antibody [3F9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50

IGF2BP2 antibody [3F9]

GTX84306
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetIGF2BP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    IGF2BP2 antibody [3F9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F9
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10644
  • Target name
    IGF2BP2
  • Target description
    insulin like growth factor 2 mRNA binding protein 2
  • Target synonyms
    IMP-2, IMP2, VICKZ2, insulin-like growth factor 2 mRNA-binding protein 2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2, VICKZ family member 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y6M1
  • Protein Name
    Insulin-like growth factor 2 mRNA-binding protein 2
  • Scientific Description
    Binds to the 5-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IMP2 using anti-IMP2 antibody (M02010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP2 antigen affinity purified monoclonal antibody (Catalog # M02010-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP2 at approximately 66 kDa. The expected band size for IMP2 is at 66 kDa.
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