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ProductsBack/IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IGF2BP2 (Cat# RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2(Cat# TA501269). Positive lysates [LY401961] (100ug) and [LC401961] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IGF2BP2 (Cat# RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2(Cat# TA501269). Positive lysates [LY401961] (100ug) and [LC401961] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IGF2BP2 (Cat# RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2(Cat# TA501269). Positive lysates [LY401961] (100ug) and [LC401961] (20ug) can be purchased separately from OriGene.

IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CF501269
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetIGF2BP2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IGF2BP2 Mouse Monoclonal Antibody [Clone ID: OTI3F9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F9
  • Gene ID10644
  • Target name
    IGF2BP2
  • Target description
    insulin like growth factor 2 mRNA binding protein 2
  • Target synonyms
    IMP-2, IMP2, VICKZ2, insulin-like growth factor 2 mRNA-binding protein 2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2, VICKZ family member 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y6M1
  • Protein Name
    Insulin-like growth factor 2 mRNA-binding protein 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) IGF2BP2 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IMP2 using anti-IMP2 antibody (M02010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP2 antigen affinity purified monoclonal antibody (Catalog # M02010-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP2 at approximately 66 kDa. The expected band size for IMP2 is at 66 kDa.
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