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ProductsBack/IGFBP3 Antibody (clone 010, Preservative Free)

IGFBP3 Antibody (clone 010, Preservative Free)

LS-C818992
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetIGFBP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IGFBP3 Antibody (clone 010, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Human IGFBP3 / IGFBP-3 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Human IGFBP3 / IGFBP-3 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    010
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3486
  • Target name
    IGFBP3
  • Target description
    insulin like growth factor binding protein 3
  • Target synonyms
    BP-53, IBP-3, IBP3, IGFBP-3, insulin-like growth factor-binding protein 3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex, binding protein 29, binding protein 53, growth hormone-dependent binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.
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