IGFBP3 antibody [84728.111]

GeneTex

IGFBP3 antibody [84728.111]

9

When used with a biotinylated anti human IGFBP3 detection antibody in sandwich ELISAs, this antibody shows less than 0.001 % cross-reactivity with recombinant human IGFI, IGFIR, IGFII, IGFBP1, IGFBP2, IGFBP4, IGFBP5, and IGFBP6.

For ELISA: Use at a concentration of 3 - 5microg/ml. Using plates coated with 100 microl/well of the capture antibody (monoclonal anti-human IGFBP3) at 4 microg/ml, in combination with 100 microl/well of a detection antibody (biotinylated IGFBP3) at 400 ng/ml, an ELISA with a range from 125 to 8000 pg/ml can be obtained for sample volumes of 100 ml. For IHC-P: Use at a concentration of 8 - 25 microg/ml on paraffin-embedded tissue sections (5 to 15 microm thick). For WB: Use at a concentration of 1 - 2 microg/ml. Detects a band of approximately 41 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

84728.111

0.5 mg/ml

Unconjugated

IGFBP3

insulin like growth factor binding protein 3

acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP-53; growth hormone-dependent binding protein; IBP3; IBP-3; IGF-binding protein 3; IGFBP-3; insulin-like growth factor-binding protein 3

Mouse

IgG2b

Insulin-like growth factor-binding protein 3

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Human

-20°C or -80°C,2°C to 8°C

12352203