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IGFBP3 antibody [HL1505]

GTX636979
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetIGFBP3
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Overview

  • Supplier
    GeneTex
  • Product Name
    IGFBP3 antibody [HL1505]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1505
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3486
  • Target name
    IGFBP3
  • Target description
    insulin like growth factor binding protein 3
  • Target synonyms
    acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP-53; growth hormone-dependent binding protein; IBP3; IBP-3; IGF-binding protein 3; IGFBP-3; insulin-like growth factor-binding protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17936
  • Protein Name
    Insulin-like growth factor-binding protein 3
  • Scientific Description
    This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.
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