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ProductsBack/IGLL1 Antibody - N-terminal region (OASG01205)

IGLL1 Antibody - N-terminal region (OASG01205)

OASG01205
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetIGLL1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    IGLL1 Antibody - N-terminal region (OASG01205)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID3543
  • Target name
    IGLL1
  • Target description
    immunoglobulin lambda like polypeptide 1
  • Target synonyms
    14.1, AGM2, CD179b, IGL1, IGL5, IGLJ14.1, IGLL, IGO, IGVPB, VPREB2, immunoglobulin lambda-like polypeptide 1, CD179 antigen-like family member B, CD179b antigen, Pre-B lymphocyte-specific protein-2, ig lambda-5, immunoglobulin omega polypeptide chain, immunoglobulin-related 14.1 protein, immunoglobulin-related protein 14.1, lambda5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IGLL1 using anti-IGLL1 antibody (A08965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGLL1 antigen affinity purified polyclonal antibody (Catalog # A08965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGLL1 at approximately 26KD. The expected band size for IGLL1 is at 23KD.
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