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ProductsBack/IHC-plus(tm) ABI1 / SSH3BP1 Antibody (aa325-375)

IHC-plus(tm) ABI1 / SSH3BP1 Antibody (aa325-375)

LS-B11045
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetABI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ABI1 / SSH3BP1 Antibody (aa325-375)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10006
  • Target name
    ABI1
  • Target description
    abl interactor 1
  • Target synonyms
    ABI-1, ABLBP4, E3B1, NAP1BP, SSH3BP, SSH3BP1, abl interactor 1, Abelson interactor 1, Abl-interactor protein 1 long, abl-binding protein 4, eps8 SH3 domain-binding protein, interactor protein AblBP4, nap1 binding protein, spectrin SH3 domain-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1/ABI1 antigen affinity purified polyclonal antibody (Catalog # PB9416) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3BP1/ABI1 at approximately 65 kDa. The expected band size for SSH3BP1/ABI1 is at 55 kDa.
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