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IHC-plus(tm) ANAPC1 / APC1 Antibody

LS-B6979
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetANAPC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ANAPC1 / APC1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64682
  • Target name
    ANAPC1
  • Target description
    anaphase promoting complex subunit 1
  • Target synonyms
    APC1, MCPR, TSG24, anaphase-promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), cyclosome subunit 1, mitotic checkpoint regulator, testis-specific gene 24 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APC1/ANAPC1 using anti-APC1/ANAPC1 antibody (A03471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC1/ANAPC1 antigen affinity purified polyclonal antibody (Catalog # A03471-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APC1/ANAPC1 at approximately 240 kDa. The expected band size for APC1/ANAPC1 is at 217 kDa.
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