IHC-plus(tm) APG12 / ATG12 Antibody (Internal)

LifeSpan BioSciences

IHC-plus(tm) APG12 / ATG12 Antibody (Internal)

23

Immunohistochemistry: LS-B2140 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2140 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction.. ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2140 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2140 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

ATG12

autophagy related 12

APG12, APG12L, FBR93, HAPG12, ubiquitin-like protein ATG12, APG12 autophagy 12-like, ATG12 autophagy related 12 homolog, Apg12 (autophagy, yeast) homolog

Rabbit

IgG

Human, Mouse

2°C to 8°C or -20°C

41116161