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ProductsBack/IHC-plus(tm) APH1A / APH-1 Antibody

IHC-plus(tm) APH1A / APH-1 Antibody

LS-B1328
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPH1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) APH1A / APH-1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 ug/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 29 kDa Observed: 28 kDa. ELISA, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 ug/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 29 kDa Observed: 28 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51107
  • Target name
    APH1A
  • Target description
    aph-1A gamma-secretase subunit
  • Target synonyms
    6530402N02Rik, APH-1, APH-1A, CGI-78, gamma-secretase subunit APH-1A, APH1A gamma secretase subunit, anterior pharynx defective 1 homolog A, aph-1 homolog A, gamma-secretase subunit, aph-1alpha, presenilin-stabilization factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APH1A using anti-APH1A antibody (A04859-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APH1A antigen affinity purified polyclonal antibody (Catalog # A04859-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APH1A at approximately 29 kDa. The expected band size for APH1A is at 29 kDa.
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