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IHC-plus(tm) APOBEC3C Antibody

LS-B14538
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAPOBEC3C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) APOBEC3C Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27350
  • Target name
    APOBEC3C
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3C
  • Target synonyms
    A3C; APOBEC1L; apolipoprotein B editing enzyme catalytic polypeptide-like 3C; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C; ARDC2; ARDC4; ARP5; bK150C2.3; DNA dC->dU-editing enzyme APOBEC-3C; PBI; phorbolin I; probable DNA dC->dU-editing enzyme APOBEC-3C
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOBEC3C using anti-APOBEC3C antibody (A07527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3C antigen affinity purified polyclonal antibody (Catalog # A07527-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3C at approximately 23 kDa. The expected band size for APOBEC3C is at 23 kDa.
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