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IHC-plus(tm) ASAH1 / Acid Ceramidase Antibody

LS-B3136
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetASAH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ASAH1 / Acid Ceramidase Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 45 kDa Observed: 49 kDa. ELISA, IF, IHC, IHC-P (2.5 µg/ml), WB (1 µg/ml) ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 45 kDa Observed: 49 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID427
  • Target name
    ASAH1
  • Target description
    N-acylsphingosine amidohydrolase 1
  • Target synonyms
    AC, ACDase, ASAH, PHP, PHP32, SMAPME, acid ceramidase, N-acylethanolamine hydrolase ASAH1, N-acylsphingosine amidohydrolase (acid ceramidase) 1, acid CDase, acylsphingosine deacylase, glycosylceramide deacylase, putative 32 kDa heart protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASAH1 antigen affinity purified polyclonal antibody (Catalog # A02055-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASAH1 at approximately 45KD. The expected band size for ASAH1 is at 45KD.
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