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IHC-plus(tm) AURKB / Aurora-B Antibody (phospho-Thr232)

LS-B22
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAURKB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) AURKB / Aurora-B Antibody (phospho-Thr232)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: LS-B22 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B22 was determined to be 2.5 ug/ml.. ELISA (1:10000 - 1:30000), IF, IHC, IHC-P (2.5 µg/ml), WB (1:250 - 1:2000) Immunohistochemistry: LS-B22 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B22 was determined to be 2.5 ug/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9212
  • Target name
    AURKB
  • Target description
    aurora kinase B
  • Target synonyms
    AIK2; AIM1; AIM-1; ARK2; ARK-2; AurB; aurkb-sv1; aurkb-sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora/IPL1-related kinase 2; aurora-1; aurora-B; aurora-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine-protein kinase 12; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; STK-1; STK12; STK5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
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