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IHC-plus(tm) BCL3 / BCL-3 Antibody

LS-B10073
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBCL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) BCL3 / BCL-3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    BCL3 antibody can be used for detection of BCL3 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 50 kDa. ELISA, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) BCL3 antibody can be used for detection of BCL3 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 50 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID602
  • Target name
    BCL3
  • Target description
    BCL3 transcription coactivator
  • Target synonyms
    BCL4, D19S37, B-cell lymphoma 3 protein, B cell CLL/lymphoma 3, B-cell leukemia/lymphoma 3, B-cell lymphoma 3-encoded protein, BCL-3, chronic lymphatic leukemia protein, proto-oncogene BCL3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of BCL3 using anti-BCL3 antibody (A02773-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL3 antigen affinity purified polyclonal antibody (Catalog # A02773-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL3 at approximately 60KD. The expected band size for BCL3 is at 60KD.
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