IHC-plus(tm) BHMT Antibody (aa391-402)

LifeSpan BioSciences

IHC-plus(tm) BHMT Antibody (aa391-402)

23

Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.. IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000), WB (0.03 - 0.1 µg/ml) Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

BHMT

betaine--homocysteine S-methyltransferase

BHMT1, HEL-S-61p, betaine--homocysteine S-methyltransferase 1, epididymis secretory sperm binding protein Li 61p, phi-crystallin

Goat

Hamster, Human, Monkey, Rat

-20°C

41116161