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ProductsBack/IHC-plus(tm) C11orf2 / Fat-Free Antibody

IHC-plus(tm) C11orf2 / Fat-Free Antibody

LS-B8850
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVPS51
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) C11orf2 / Fat-Free Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Fat Free antibody can be used for detection of Fat Free by Western blot at 1 ug/mL. Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Western Blot: 86 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) Fat Free antibody can be used for detection of Fat Free by Western blot at 1 ug/mL. Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Western Blot: 86 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID738
  • Target name
    VPS51
  • Target description
    VPS51 subunit of GARP complex
  • Target synonyms
    ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13, vacuolar protein sorting-associated protein 51 homolog, VPS51, GARP complex subunit, another new gene 2 protein, protein fat-free homolog, vacuolar protein sorting 51 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FFR/VPS51 using anti-FFR/VPS51 antibody (A08518-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFR/VPS51 antigen affinity purified polyclonal antibody (Catalog # A08518-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FFR/VPS51 at approximately 86 kDa. The expected band size for FFR/VPS51 is at 86 kDa.
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