IHC-plus(tm) CASZ1 Antibody

LifeSpan BioSciences

IHC-plus(tm) CASZ1 Antibody

23

A BLAST analysis was used to suggest cross reactivity with Cas5 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5

Immunohistochemistry: LS-B2838 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2838 was determined to be 5 ug/ml. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.. ELISA (1:10000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B2838 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2838 was determined to be 5 ug/ml. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

CASZ1

castor zinc finger 1

CAS11; castor homolog 1, zinc finger; castor-related protein; CST; dJ734G22.1; SRG; survival-related; zinc finger protein 693; zinc finger protein castor homolog 1; ZNF693

Rabbit

IgG

Bovine, Human, Mouse, Primate, Rat

12352203