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ProductsBack/IHC-plus(tm) CCDC88A / GIV / Girdin Antibody

IHC-plus(tm) CCDC88A / GIV / Girdin Antibody

LS-B5063
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCDC88A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CCDC88A / GIV / Girdin Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Girdin antibody can be used for detection of Girdin by Western blot at 1 - 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) Girdin antibody can be used for detection of Girdin by Western blot at 1 - 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55704
  • Target name
    CCDC88A
  • Target description
    coiled-coil and HOOK domain protein 88A
  • Target synonyms
    APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL, girdin, AKT-phosphorylation enhancer, Hook-related protein 1, coiled-coil domain containing 88A, g alpha-interacting vesicle-associated protein, girders of actin filaments
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCDC88A using anti-CCDC88A antibody (A03282-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC88A antigen affinity purified polyclonal antibody (Catalog # A03282-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC88A at approximately 240 kDa. The expected band size for CCDC88A is at 216 kDa.
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