IHC-plus(tm) CCL5 / RANTES Antibody (clone 8.PP.11)
LS-B2472
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCL5
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) CCL5 / RANTES Antibody (clone 8.PP.11)
- Delivery Days Customer23
- Antibody SpecificityRecognizes human RANTES.
- Application Supplier NoteImmunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml).. ELISA (2 - 4 µg/ml), IHC, IHC-P (5 µg/ml) Immunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml).
- ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8.PP.11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID6352
- Target nameCCL5
- Target descriptionC-C motif chemokine ligand 5
- Target synonymsbeta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; D17S136E; eoCP; eosinophil chemotactic cytokine; RANTES; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SISd; SIS-delta; small inducible cytokine subfamily A (Cys-Cys), member 5; T-cell specific protein p288; TCP228
- HostMouse
- IsotypeIgG2b
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203