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IHC-plus(tm) CCL5 / RANTES Antibody (clone 8.PP.11)

LS-B2472
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCCL5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CCL5 / RANTES Antibody (clone 8.PP.11)
  • Delivery Days Customer
    23
  • Antibody Specificity
    Recognizes human RANTES.
  • Application Supplier Note
    Immunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml).. ELISA (2 - 4 µg/ml), IHC, IHC-P (5 µg/ml) Immunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml).
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8.PP.11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6352
  • Target name
    CCL5
  • Target description
    C-C motif chemokine ligand 5
  • Target synonyms
    beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; D17S136E; eoCP; eosinophil chemotactic cytokine; RANTES; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SISd; SIS-delta; small inducible cytokine subfamily A (Cys-Cys), member 5; T-cell specific protein p288; TCP228
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203