IHC-plus(tm) CCL8 / MCP2 Antibody

LifeSpan BioSciences

IHC-plus(tm) CCL8 / MCP2 Antibody

23

Immunohistochemistry: LS-B8198 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-2 when used with biotinylated antigen affinity purified anti-human MCP-2 (LS-C104731) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.. ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml) Immunohistochemistry: LS-B8198 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-2 when used with biotinylated antigen affinity purified anti-human MCP-2 (LS-C104731) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

CCL8

C-C motif chemokine ligand 8

HC14, MCP-2, MCP2, SCYA10, SCYA8, C-C motif chemokine 8, chemokine (C-C motif) ligand 8, monocyte chemoattractant protein 2, monocyte chemotactic protein 2, small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2), small-inducible cytokine A8

Mouse

IgG1

Human

-20°C,2°C to 8°C,RT

41116161