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IHC-plus(tm) CDK13 / CDC2L5 Antibody

LS-B14812
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCDK13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CDK13 / CDC2L5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 158kDa/164kDa, while the observed MW by Western blot was 210kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:1000 - 1:2000) The predicted MW is 158kDa/164kDa, while the observed MW by Western blot was 210kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.39 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8621
  • Target name
    CDK13
  • Target description
    cyclin dependent kinase 13
  • Target synonyms
    CDC2L, CDC2L5, CHDFIDD, CHED, hCDK13, cyclin-dependent kinase 13, CDC2-related protein kinase 5, cell division cycle 2-like protein kinase 5, cell division protein kinase 13, cholinesterase-related cell division controller
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CDK13 using anti-CDK13 antibody (A05292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK13 antigen affinity purified polyclonal antibody (Catalog # A05292-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK13 at approximately 185-190KD. The expected band size for CDK13 is at 165KD.
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