IHC-plus(tm) CDK2 Antibody

LifeSpan BioSciences

IHC-plus(tm) CDK2 Antibody

23

Recognizes human Cdk2. Species cross-reactivity: mouse and rat.

Immunohistochemistry: LS-B2482 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2482 was determined to be 5 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 ug/ml for 2hrs at RT. Positive control: HeLa cells.. ELISA, IF, IHC, IHC-P (5 µg/ml), IP, WB (1 - 2 µg/ml) Immunohistochemistry: LS-B2482 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2482 was determined to be 5 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 ug/ml for 2hrs at RT. Positive control: HeLa cells.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.1 mg/ml

Unconjugated

...

CDK2

cyclin dependent kinase 2

cdc2-related protein kinase; CDKN2; cell division protein kinase 2; cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2)

Mouse

IgG2a, IgG2b

Human, Mouse, Rat

2°C to 8°C or -20°C

12352203