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ProductsBack/IHC-plus(tm) CRTC2 / TORC2 Antibody (aa83-96)

IHC-plus(tm) CRTC2 / TORC2 Antibody (aa83-96)

LS-B9228
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
TargetCRTC2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CRTC2 / TORC2 Antibody (aa83-96)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 85kD band observed in lysates of cell line Jurkat (calculated MW of 73.3kD according to NP_859066.1). Recommended concentration: 0.3-1 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 85kD band observed in lysates of cell line Jurkat (calculated MW of 73.3kD according to NP_859066.1). Recommended concentration: 0.3-1 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID200186
  • Target name
    CRTC2
  • Target description
    CREB regulated transcription coactivator 2
  • Target synonyms
    TORC-2, TORC2, CREB-regulated transcription coactivator 2, transducer of regulated cAMP response element-binding protein (CREB) 2
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TORC2 using anti-TORC2 antibody (A01118-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC2 antigen affinity purified polyclonal antibody (Catalog # A01118-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC2 at approximately 73KD. The expected band size for TORC2 is at 73KD.
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