IHC-plus(tm) CTGF Antibody

LifeSpan BioSciences

IHC-plus(tm) CTGF Antibody

23

Human CTGF

Immunohistochemistry: LS-B7479 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCTGF (5 ug/ml), a concentration of 6-9 ug/ml of this antibody is required. ELISA: To detect hCTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (LS-C104563) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7479 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCTGF (5 ug/ml), a concentration of 6-9 ug/ml of this antibody is required. ELISA: To detect hCTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (LS-C104563) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

1 mg/ml

...

Lyophilized

CCN2

cellular communication network factor 2

CCN family member 2; connective tissue growth factor; CTGF; HCS24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; insulin-like growth factor-binding protein 8; NOV2

-20°C,2°C to 8°C,RT

12352203