IHC-plus(tm) CTGF Antibody

LifeSpan BioSciences

IHC-plus(tm) CTGF Antibody

23

Immunohistochemistry: LS-B7479 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCTGF (5 ug/ml), a concentration of 6-9 ug/ml of this antibody is required. ELISA: To detect hCTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (LS-C104563) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7479 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCTGF (5 ug/ml), a concentration of 6-9 ug/ml of this antibody is required. ELISA: To detect hCTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (LS-C104563) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGF. Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

CCN2

cellular communication network factor 2

CTGF, HCS24, IBP-8, IGFBP8, KMD, NOV2, SEMDLSL, CCN family member 2, IGF-binding protein 8, connective tissue growth factor, hypertrophic chondrocyte-specific protein 24, insulin-like growth factor-binding protein 8

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

12352203