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IHC-plus(tm) CUX1 / CASP Antibody

LS-B13869
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCUX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CUX1 / CASP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 72-77kDa/147-165kDa, while the observed MW by Western blot was 88kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:1000 - 1:2000) The predicted MW is 72-77kDa/147-165kDa, while the observed MW by Western blot was 88kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.97 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1523
  • Target name
    CUX1
  • Target description
    cut like homeobox 1
  • Target synonyms
    CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, NEDDMS, Nbla10317, p100, p110, p200, p75, protein CASP, Homeobox protein cut-like 1, CCAAT displacement protein, CDP/Cux p200, CUX1 gene Alternatively Spliced Product, cut homolog, golgi integral membrane protein 6, homeobox protein cux-1, mutant CUX1, putative protein product of Nbla10317
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CUX1 using anti-CUX1 antibody (A01653-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUX1 antigen affinity purified polyclonal antibody (Catalog # A01653-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CUX1 at approximately 200 kDa. The expected band size for CUX1 is at 164 kDa.
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