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ProductsBack/IHC-plus(tm) CYP1A1 Antibody

IHC-plus(tm) CYP1A1 Antibody

LS-B12899
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYP1A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CYP1A1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 18kDa/20kDa/58kDa, while the observed MW by Western blot was 63kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 18kDa/20kDa/58kDa, while the observed MW by Western blot was 63kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1543
  • Target name
    CYP1A1
  • Target description
    cytochrome P450 family 1 subfamily A member 1
  • Target synonyms
    AHH, AHRR, CP11, CYP1, CYPIA1, P1-450, P450-C, P450DX, cytochrome P450 1A1, aryl hydrocarbon hydroxylase, cytochrome P1-450, dioxin-inducible, cytochrome P450 form 6, cytochrome P450, family 1, subfamily A, polypeptide 1, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1, cytochrome P450-C, cytochrome P450-P1, flavoprotein-linked monooxygenase, hydroperoxy icosatetraenoate dehydratase, xenobiotic monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Lung Tissue Lysate Lane 2: Mouse Lung Tissue Lysate Lane 3: Human Placenta Tissue Lysate Lane 4: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD.
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