IHC-plus(tm) DAXX Antibody

LifeSpan BioSciences

IHC-plus(tm) DAXX Antibody

23

A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Immunohistochemistry: LS-B363 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B363 was determined to be 2.5 ug/ml.. ELISA (1:7000 - 1:45000), IF, IHC, IHC-P (2.5 µg/ml), WB (1:200 - 1:2000) Immunohistochemistry: LS-B363 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B363 was determined to be 2.5 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.06 mg/ml

Unconjugated

...

DAXX

death domain associated protein

BING2; CENP-C binding protein; DAP6; death domain-associated protein 6; death-associated protein 6; EAP1; ETS1-associated protein 1; fas death domain-associated protein; Fas-binding protein; SMIM40

Rabbit

IgG

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203