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IHC-plus(tm) DDIT3 / CHOP Antibody

LS-B12888
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDIT3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) DDIT3 / CHOP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 19kDa/21kDa, while the observed MW by Western blot was 26kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 19kDa/21kDa, while the observed MW by Western blot was 26kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1649
  • Target name
    DDIT3
  • Target description
    DNA damage inducible transcript 3
  • Target synonyms
    AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP zeta, CCAAT/enhancer-binding protein homologous protein, alternative DDIT3 protein, c/EBP-homologous protein 10, growth arrest and DNA damage-inducible protein GADD153
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT3 antigen affinity purified polyclonal antibody (Catalog # A00311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDIT3 at approximately 29KD. The expected band size for DDIT3 is at 29KD.
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