IHC-plus(tm) E2F1 Antibody (phospho-Ser364)

LifeSpan BioSciences

IHC-plus(tm) E2F1 Antibody (phospho-Ser364)

23

Immunohistochemistry: LS-B52 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B52 was determined to be 10 ug/ml.. ELISA (1:20000 - 1:100000), IHC, IHC-P (10 µg/ml), WB (1:250 - 1:2000) Immunohistochemistry: LS-B52 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B52 was determined to be 10 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

E2F1

E2F transcription factor 1

E2F-1, RBAP1, RBBP3, RBP3, transcription factor E2F1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, retinoblastoma-binding protein 3 (E2F-like)

Rabbit

IgG

Human, Primate

2°C to 8°C or -20°C

41116161