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ProductsBack/IHC-plus(tm) EED Antibody

IHC-plus(tm) EED Antibody

LS-B13877
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEED
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) EED Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 45kDa/50kDa/53kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 45kDa/50kDa/53kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8726
  • Target name
    EED
  • Target description
    embryonic ectoderm development
  • Target synonyms
    COGIS; embryonic ectoderm development protein; HEED; polycomb protein EED; WAIT1; WD protein associating with integrin cytoplasmic tails 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EED using anti-EED antibody (A01345-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (Catalog # A01345-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EED at approximately 70 kDa. The expected band size for EED is at 50 kDa.
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