IHC-plus(tm) FHIT Antibody

LifeSpan BioSciences

IHC-plus(tm) FHIT Antibody

23

Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stom

Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.. ELISA, IHC, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

FHIT

fragile histidine triad diadenosine triphosphatase

AP3A hydrolase; AP3Aase; bis(5'-adenosyl)-triphosphatase; diadenosine 5',5'''-P1,P3-triphosphate hydrolase; dinucleosidetriphosphatase; FRA3B

Rabbit

IgG

Human, Rat

-20°C,2°C to 8°C

12352203