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ProductsBack/IHC-plus(tm) FOXO3 / FOXO3A Antibody

IHC-plus(tm) FOXO3 / FOXO3A Antibody

LS-B5284
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXO3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) FOXO3 / FOXO3A Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 4 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 4 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2309
  • Target name
    FOXO3
  • Target description
    forkhead box O3
  • Target synonyms
    AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, forkhead box protein O3, FOXO3A-, forkhead box O3A, forkhead homolog (rhabdomyosarcoma) like 1, forkhead in rhabdomyosarcoma-like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (A00252-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO3A/FOXO3 antigen affinity purified polyclonal antibody (Catalog # A00252-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO3A/FOXO3 at approximately 90 kDa. The expected band size for FOXO3A/FOXO3 is at 71,80-100 kDa.
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