More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) GAD65 Antibody (aa515-528)

IHC-plus(tm) GAD65 Antibody (aa515-528)

LS-B3937
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetGAD2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) GAD65 Antibody (aa515-528)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 1.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2572
  • Target name
    GAD2
  • Target description
    glutamate decarboxylase 2
  • Target synonyms
    GAD65, glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • Host
    Goat
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-GAD2 Antibody144-00971
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAD2
  • SizePrice
Figure 1. Western blot analysis of GAD65 using anti-GAD65 antibody (A03142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65 antigen affinity purified polyclonal antibody (Catalog # A03142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD65 at approximately 65 kDa. The expected band size for GAD65 is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-GAD65/GAD2 Antibody Picoband(r)A03142-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAD2
  • SizePrice
GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term (GTX100281) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
GeneTex
GAD65 antibody [C2C3], C-termGTX100281
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGAD2
  • SizePrice
Product group Antibodies
Biomatik
Gad2 Polyclonal AntibodyCAC08038
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetGAD2
  • SizePrice
Product group Antibodies
Assay Genie
GAD2 AntibodyCAB0971
ReactivityHuman
TargetGAD2
  • SizePrice
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss
GAD65 Polyclonal AntibodyBS-0400R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
TargetGAD2
  • SizePrice
Anti-GAD2 Antibody
Product group Antibodies
Antibodies.com
Anti-GAD2 AntibodyA28907
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-GAD2 / GAD65EB06730
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetGAD2
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91048 antibody. Corresponding GAD2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-GAD2 AntibodyAMAB91048
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAD2
  • SizePrice
Product group Antibodies
MedChem Express
GAD65 Antibody (YA758, Mouse)HY-P80682
TargetGAD2
  • SizePrice