SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
GAD65 Polyclonal Antibody
BS-0400R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAD2
Overview
- SupplierBioss Antibodies
- Product NameGAD65 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2572
- Target nameGAD2
- Target descriptionglutamate decarboxylase 2
- Target synonyms65 kDa glutamic acid decarboxylase; GAD65; GAD-65; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase-2 (pancreas)
- HostRabbit
- IsotypeIgG
- Protein IDQ05329
- Protein NameGlutamate decarboxylase 2
- Storage Instruction-20°C
- UNSPSC12352203
References
- Striatal and Tegmental Neurons Code Critical Signals for Temporal-Difference Learning of State Value in Domestic Chicks. Wen C et al., 2016, Front NeurosciRead more
Datasheet
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