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ProductsBack/IHC-plus(tm) Geminin Antibody (clone 1A8)

IHC-plus(tm) Geminin Antibody (clone 1A8)

LS-B6226
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGMNN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Geminin Antibody (clone 1A8)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.. ELISA, IF (10 µg/ml), IHC, IHC-P (1 - 5 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1A8
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51053
  • Target name
    GMNN
  • Target description
    geminin DNA replication inhibitor
  • Target synonyms
    Gem, MGORS6, geminin
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Geminin/GMNN using anti-Geminin/GMNN antibody (A06060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Geminin/GMNN antigen affinity purified polyclonal antibody (Catalog # A06060-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Geminin/GMNN at approximately 25 kDa. The expected band size for Geminin/GMNN is at 25 kDa.
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