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ProductsBack/IHC-plus(tm) GRIN1 / NMDAR1 Antibody

IHC-plus(tm) GRIN1 / NMDAR1 Antibody

LS-B13901
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) GRIN1 / NMDAR1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 99kDa/101kDa/103kDa/105kDa/106kDa/107kDa, while the observed MW by Western blot was 105kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 99kDa/101kDa/103kDa/105kDa/106kDa/107kDa, while the observed MW by Western blot was 105kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2902
  • Target name
    GRIN1
  • Target description
    glutamate ionotropic receptor NMDA type subunit 1
  • Target synonyms
    DEE101, GluN1, MRD8, NDHMSD, NDHMSR, NMD-R1, NMDA1, NMDAR1, NR1, hNR1, glutamate receptor ionotropic, NMDA 1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, glutamate [NMDA] receptor subunit zeta-1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, putative NMDtranscript(altAcc_e2)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NMDAR1 using anti-NMDAR1 antibody (A01808). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR1 antigen affinity purified polyclonal antibody (Catalog # A01808) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR1 at approximately 120KD. The expected band size for NMDAR1 is at 105KD.
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