IHC-plus(tm) GSN / Gelsolin Antibody

LifeSpan BioSciences

IHC-plus(tm) GSN / Gelsolin Antibody

23

Immunohistochemistry: LS-B2480 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2480 was determined to be 5 ug/ml. ELISA: 1:2000 applies to gelsolin presented on a coat of polyclonal capture antibody. Note: Does not work as capture antibody in sandwich ELISA.. ELISA (1:2000), IHC, IHC-P (5 µg/ml) Immunohistochemistry: LS-B2480 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2480 was determined to be 5 ug/ml. ELISA: 1:2000 applies to gelsolin presented on a coat of polyclonal capture antibody. Note: Does not work as capture antibody in sandwich ELISA.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1.03 mg/ml

Unconjugated

GSN

gelsolin

ADF, AGEL, AMYLD4, gelsolin, actin-depolymerizing factor, brevin, epididymis secretory sperm binding protein

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161