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ProductsBack/IHC-plus(tm) GSTA1 Antibody (clone 2F7)

IHC-plus(tm) GSTA1 Antibody (clone 2F7)

LS-B6091
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGSTA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) GSTA1 Antibody (clone 2F7)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F7
  • Conjugate
    Unconjugated
  • Gene ID2938
  • Target name
    GSTA1
  • Target description
    glutathione S-transferase alpha 1
  • Target synonyms
    GST-epsilon, GST2, GSTA1-1, GTH1, glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, S-(hydroxyalkyl)glutathione lyase A1, androst-5-ene-3,17-dione isomerase, glutathione S-alkyltransferase A1, glutathione S-aryltransferase A1, glutathione S-transferase 2, glutathione S-transferase Ha subunit 1, testicular tissue protein Li 80
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of GSTA1/A2/A3/A4/A5 using anti-GSTA1/A2/A3/A4/A5 antibody (A01462-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HUH-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTA1/A2/A3/A4/A5 antigen affinity purified polyclonal antibody (Catalog # A01462-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTA1/A2/A3/A4/A5 at approximately 26 kDa. The expected band size for GSTA1/A2/A3/A4/A5 is at 26 kDa.
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