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ProductsBack/IHC-plus(tm) HAO1 Antibody (clone UMAB117)

IHC-plus(tm) HAO1 Antibody (clone UMAB117)

LS-B16924
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetHAO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HAO1 Antibody (clone UMAB117)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Applications Supplier
    IHC-P (1:50), PMA, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    UMAB117
  • Conjugate
    Unconjugated
  • Gene ID54363
  • Target name
    HAO1
  • Target description
    hydroxyacid oxidase 1
  • Target synonyms
    GO, GOX, GOX1, HAOX1, 2-Hydroxyacid oxidase 1, (S)-2-hydroxy-acid oxidase, glycolate oxidase 1, glyoxylate oxidase, hydroxyacid oxidase (glycolate oxidase) 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HAO1 using anti-HAO1 antibody (A09159-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO1 antigen affinity purified polyclonal antibody (Catalog # A09159-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO1 at approximately 41 kDa. The expected band size for HAO1 is at 41 kDa.
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