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ProductsBack/IHC-plus(tm) HCCS Antibody (aa81-130)

IHC-plus(tm) HCCS Antibody (aa81-130)

LS-B10025
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetHCCS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HCCS Antibody (aa81-130)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 30 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    IF (1:100 - 1:500), IHC, IHC-Fr (1:200), Peptide-ELISA (1:20000), WB (1:500 - 1:1000) Western Blot: Predicted MW 30 kDa IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3052
  • Target name
    HCCS
  • Target description
    holocytochrome c synthase
  • Target synonyms
    CCHL, LSDMCA1, MCOPS7, MLS, holocytochrome c-type synthase, cytochrome c heme-lyase, cytochrome c-type heme lyase, holocytochrome-c synthetase, microphthalamia with linear skin defects, microphthalmia with linear skin defects
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HCCS using anti-HCCS antibody (A00924-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCCS antigen affinity purified polyclonal antibody (Catalog # A00924-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCCS at approximately 31 kDa. The expected band size for HCCS is at 31 kDa.
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