IHC-plus(tm) HIF1AN Antibody (C-Terminus)

LifeSpan BioSciences

IHC-plus(tm) HIF1AN Antibody (C-Terminus)

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Immunohistochemistry: LS-B667 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B667 was determined to be 5 ug/ml.. IHC, IHC-P (5 µg/ml), IP, WB (1:1000 - 1:5000) Immunohistochemistry: LS-B667 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B667 was determined to be 5 ug/ml.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

HIF1AN

hypoxia inducible factor 1 subunit alpha inhibitor

FIH1, hypoxia-inducible factor 1-alpha inhibitor, FIH-1, factor inhibiting HIF-1, factor inhibiting HIF1, hypoxia inducible factor 1 alpha subunit inhibitor, hypoxia-inducible factor asparagine hydroxylase, peptide-aspartate beta-dioxygenase

Rabbit

Human, Mouse, Rat

2°C to 8°C

41116161