IHC-plus(tm) IRF3 Antibody

LifeSpan BioSciences

IHC-plus(tm) IRF3 Antibody

23

Detects endogenous levels of human IRF3 at 51kD mouse and rat, at 57kD human. Species cross-reactivity: mouse, rat and monkey.

Immunohistochemistry: LS-B2529 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2529 was determined to be 1:50.. IHC, IHC-P (1:50), WB (1:1000) Immunohistochemistry: LS-B2529 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2529 was determined to be 1:50.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

IRF3

interferon regulatory factor 3

IIAE7; interferon regulatory factor 3

Rabbit

IgG

Human, Monkey

-20°C,2°C to 8°C

12352203