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ProductsBack/IHC-plus(tm) ITCH / AIP4 Antibody

IHC-plus(tm) ITCH / AIP4 Antibody

LS-B15714
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetITCH
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ITCH / AIP4 Antibody
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:25 - 1:100), ICC (1:20 - 1:100), IF (1:20 - 1:100), IHC, IHC-P (1:50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83737
  • Target name
    ITCH
  • Target description
    itchy E3 ubiquitin protein ligase
  • Target synonyms
    ADMFD, AIF4, AIP4, NAPP1, E3 ubiquitin-protein ligase Itchy homolog, HECT-type E3 ubiquitin transferase Itchy homolog, NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, itchy E3 ubiquitin protein ligase homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ITCH using anti-ITCH antibody (A00195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH antigen affinity purified polyclonal antibody (Catalog # A00195-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITCH at approximately 103KD. The expected band size for ITCH is at 103KD.
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