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ProductsBack/IHC-plus(tm) LDLR / LDL Receptor Antibody (Internal)

IHC-plus(tm) LDLR / LDL Receptor Antibody (Internal)

LS-B10039
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetLDLR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LDLR / LDL Receptor Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3949
  • Target name
    LDLR
  • Target description
    low density lipoprotein receptor
  • Target synonyms
    FH, FHC, FHCL1, LDLCQ2, low-density lipoprotein receptor, LDL receptor, low-density lipoprotein receptor class A domain-containing protein 3
  • Host
    Rabbit
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LDLR using anti-LDLR antibody (A00076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDLR antigen affinity purified polyclonal antibody (Catalog # A00076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDLR at approximately 130KD. The expected band size for LDLR is at 95KD.
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HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ LDL receptor Polyclonal Antibody(bs-0705R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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