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ProductsBack/IHC-plus(tm) LMTK3 Antibody (aa1251-1300)

IHC-plus(tm) LMTK3 Antibody (aa1251-1300)

LS-B9975
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLMTK3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LMTK3 Antibody (aa1251-1300)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 153 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), Peptide-ELISA (1:20000), WB (1:500 - 1:1000) Western Blot: Predicted MW 153 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID114783
  • Target name
    LMTK3
  • Target description
    lemur tyrosine kinase 3
  • Target synonyms
    LMR3; PPP1R101; protein phosphatase 1, regulatory subunit 101; serine/threonine-protein kinase LMTK3; TYKLM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LMTK3 using anti-LMTK3 antibody (A08432-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human U2OS whole cell lysates Lane 4: mouse thymus tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMTK3 antigen affinity purified polyclonal antibody (Catalog # A08432-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMTK3 at approximately 153KD. The expected band size for LMTK3 is at 153KD.
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