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ProductsBack/IHC-plus(tm) LPAR3 / LPA3 / EDG7 Antibody (Internal)

IHC-plus(tm) LPAR3 / LPA3 / EDG7 Antibody (Internal)

LS-A1016
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLPAR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LPAR3 / LPA3 / EDG7 Antibody (Internal)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.. ELISA, IHC, IHC-P (5 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23566
  • Target name
    LPAR3
  • Target description
    lysophosphatidic acid receptor 3
  • Target synonyms
    EDG7, Edg-7, GPCR, HOFNH30, LP-A3, LPA3, RP4-678I3, lysophosphatidic acid receptor 3, LPA receptor 3, LPA receptor EDG7, LPA-3, calcium-mobilizing lysophosphatidic acid receptor LP-A3, endothelial cell differentiation gene 7, endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7, lysophosphatidic acid receptor Edg-7
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDG7/LPAR3 using anti-EDG7/LPAR3 antibody (A06597-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG7/LPAR3 antigen affinity purified polyclonal antibody (Catalog # A06597-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG7/LPAR3 at approximately 43 kDa. The expected band size for EDG7/LPAR3 is at 40 kDa.
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