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ProductsBack/IHC-plus(tm) LYZ / Lysozyme Antibody

IHC-plus(tm) LYZ / Lysozyme Antibody

LS-B11839
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLYZ
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LYZ / Lysozyme Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Paraffin and frozen. Control tissue: Tonsil, Lymph Node. Localization: Cytoplasmic.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-Fr (1:100 - 1:400), IHC-P (1:100) Immunohistochemistry: Paraffin and frozen. Control tissue: Tonsil, Lymph Node. Localization: Cytoplasmic.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4069
  • Target name
    LYZ
  • Target description
    lysozyme
  • Target synonyms
    AMYLD5, LYZF1, LZM, lysozyme C, 1,4-beta-N-acetylmuramidase C, c-type lysozyme, lysozyme F1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (A01811-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (Catalog # A01811-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.
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