IHC-plus(tm) MMP9 / Gelatinase B Antibody

LifeSpan BioSciences

IHC-plus(tm) MMP9 / Gelatinase B Antibody

23

Immunohistochemistry: LS-B2572 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2572 was determined to be 10 ug/ml. Inhibition of MMP-9 Activity: 2-4 ug/ml (order Ab without sodium azide). Immunoprecipitation (Protein G): 2 ug/mg protein lysate (Native verified). Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells.. ELISA, IHC, IHC-Fr, IHC-P (10 µg/ml), IP Immunohistochemistry: LS-B2572 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2572 was determined to be 10 ug/ml. Inhibition of MMP-9 Activity: 2-4 ug/ml (order Ab without sodium azide). Immunoprecipitation (Protein G): 2 ug/mg protein lysate (Native verified). Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells.

ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.1 mg/ml

Unconjugated

MMP9

matrix metallopeptidase 9

CLG4B, GELB, MANDP2, MMP-9, matrix metalloproteinase-9, macrophage gelatinase, matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), type V collagenase

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161