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IHC-plus(tm) MOG Antibody

LS-A12576
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMOG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MOG Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1.25 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4340
  • Target name
    MOG
  • Target description
    myelin oligodendrocyte glycoprotein
  • Target synonyms
    BTN6, BTNL11, MOGIG2, NRCLP7, myelin-oligodendrocyte glycoprotein, MOG AluA, MOG AluB, MOG Ig-AluB, MOG alpha-5
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin oligodendrocyte glycoprotein antigen affinity purified polyclonal antibody (Catalog # A03294) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin oligodendrocyte glycoprotein at approximately 26KD. The expected band size for Myelin oligodendrocyte glycoprotein is at 28KD.
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